ARG22278

anti-Cav 1.3 antibody [S48A-9]

anti-Cav 1.3 antibody [S48A-9] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S48A-9] recognizes Cav 1.3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~250kDa. No cross-reactivity against Cav1.2.
Host Mouse
Clonality Monoclonal
Clone S48A-9
Isotype IgG2a, kappa
Target Name Cav 1.3
Antigen Species Rat
Immunogen Fusion protein around aa. 859-875 of Rat CaV1.3
Conjugation Un-conjugated
Alternate Names SANDD; CCHL1A2; Cav1.3; CACN4; PASNA; CACNL1A2; Calcium channel, L type, alpha-1 polypeptide, isoform 2; Voltage-dependent L-type calcium channel subunit alpha-1D; CACH3; Voltage-gated calcium channel subunit alpha Cav1.3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC1:1000
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12289 Mouse CACNA1D

GeneID: 29716 Rat CACNA1D

GeneID: 776 Human CACNA1D

Gene Symbol Cacna1d
Gene Full Name calcium channel, voltage-dependent, L type, alpha 1D subunit
Background Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
Function Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). [UniProt]
Cellular Localization Cell membrane, Membrane
Calculated MW 245 kDa