ARG46832
anti-ChAT antibody
anti-ChAT antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ChAT |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ChAT |
| Immunogen | Recombinant protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 71 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen |
| Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide |
| Preservative | Proclin 300 or Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHAT |
| Gene Full Name | choline O-acetyltransferase |
| Background | Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999). |
| Calculated MW | 83 kDa |
Images (7) Click the Picture to Zoom In
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ARG46832 anti-ChAT antibody IHC-P image
Immunohistochemistry: Human liver stained with ARG46832 anti-ChAT antibody at 1:200 dilution.
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ARG46832 anti-ChAT antibody ICC/IF image
Immunofluorescence: THP-1 stained with ARG46832 anti-ChAT antibody at 1:100 dilution.
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ARG46832 anti-ChAT antibody WB image
Western blot: Hela stained with ARG46832 anti-ChAT antibody at 1:1000 dilution.
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ARG46832 anti-ChAT antibody ICC/IF image
Immunofluorescence: NIH/3T3 stained with ARG46832 anti-ChAT antibody at 1:100 dilution.
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ARG46832 anti-ChAT antibody WB image
Western blot: C6 stained with ARG46832 anti-ChAT antibody at 1:1000 dilution.
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ARG46832 anti-ChAT antibody IHC-P image
Immunohistochemistry: Mouse lung stained with ARG46832 anti-ChAT antibody at 1:200 dilution.
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ARG46832 anti-ChAT antibody WB image
Western blot: Mouse brain and mouse spinal cord stained with ARG46832 anti-ChAT antibody at 1:2500 dilution.
