ARG52242

anti-ChAT antibody

anti-ChAT antibody for IHC-Frozen sections,Western blot and Chicken,Guinea pig,Human,Mouse,Primates,Rat

Neuroscience antibody

Overview

Product Description

Goat Polyclonal antibody recognizes ChAT

Tested Reactivity Hu, Ms, Rat, Chk, Gpig, NHuPrm
Tested Application IHC-Fr, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ChAT
Antigen Species Human
Immunogen Native choline acetyltransferase purifed from human placenta
Conjugation Un-conjugated
Alternate Names CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:1,00
WB1:1,00
Application Note Specific for the ~ 70k choline acetyltransferase protein
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1103 Human CHAT

GeneID: 12647 Mouse CHAT

GeneID: 395314 Chicken CHAT

Gene Symbol CHAT
Gene Full Name choline O-acetyltransferase
Background Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Research Area Neuroscience antibody
Calculated MW 83 kDa

Images (1) Click the Picture to Zoom In

  • ARG52242 anti-ChAT antibody WB image

    Western blot: rat brain lysate stained with ARG52242 anti-ChAT antibody showing specific immunolabeling of the ~70k ChAT.