ARG67281

anti-Choline Acetyltransferase antibody

anti-Choline Acetyltransferase antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Monoclonal antibody recognizes Choline Acetyltransferase
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Isotype IgG,Kappa
Target Name Choline Acetyltransferase
Conjugation Un-conjugated
Alternate Names CHAT; Choline O-Acetyltransferase ; CHOACTase; Choline Acetylase; EC 2.3.1.6; Acetyl CoA:Choline O-Acetyltransferase; Choline Acetyltransferase; EC 2.3.1; CMS1A2; CMS1A; CMS6; ChAT

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
IP1:50 - 1:200
WB1:2000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A
Buffer PBS, 0.05% Proclin 300, 50% glycerol and 0.05% BSA
Preservative 0.05% Proclin 300
Stabilizer 50% glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1103 Human CHAT

GeneID: 12647 Mouse CHAT

Swiss-port # P28329 Human Choline O-acetyltransferase

Gene Symbol CHAT
Gene Full Name Choline O-Acetyltransferase
Background This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. [UniProt]
Calculated MW 83 kDa

Images (3) Click the Picture to Zoom In

  • ARG67281 anti-Choline Acetyltransferase antibody WB image

    Western blot: SH-SY5Y stained with ARG67281 anti-Choline Acetyltransferase antibody.

  • ARG67281 anti-Choline Acetyltransferase antibody WB image

    Western blot: Mouse brain stained with ARG67281 anti-Choline Acetyltransferase antibody.

  • ARG67281 anti-Choline Acetyltransferase antibody WB image

    Western blot: Rat brain stained with ARG67281 anti-Choline Acetyltransferase antibody.