ARG20528

anti-Cofilin phospho (Ser3) antibody

anti-Cofilin phospho (Ser3) antibody for ELISA,ICC/IF,Western blot and Chicken,Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication5

Overview

Product Description Rabbit Polyclonal antibody recognizes Cofilin phospho (Ser3)
Tested Reactivity Hu, Ms, Rat, Chk
Tested Application ELISA, ICC/IF, WB
Specificity This antibody was cross-adsorbed to unphosphorylated Coflin 1 peptide then affinity purified using phospho-Cofilin 1 (Ser-3) peptide (without carrier). The antibody detects a ~19 kDa protein corresponding to the molecular mass of phosphorylated Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment.
Host Rabbit
Clonality Polyclonal
Target Name Cofilin
Antigen Species Human
Immunogen KLH-conjugated phosphospecific peptide around Ser3 in human Cofilin 1 protein. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
Conjugation Un-conjugated
Full Name cofilin 1 (non-muscle)
Alternate Names CFL; 18 kDa phosphoprotein; Cofilin, non-muscle isoform; HEL-S-15; p18; cofilin; Cofilin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000
ICC/IF1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 50% Glycerol, 1 mg/ml BSA, and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 50% Glycerol, 1 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1072 Human CFL1

GeneID: 12631 Mouse CFL1

GeneID: 29271 Rat CFL1

Gene Symbol CFL1
Gene Full Name cofilin 1 (non-muscle)
Background Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (Cofilin 2), non-muscle cofilin (Cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
PTM Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

Images (1) Click the Picture to Zoom In

  • ARG20528 anti-Cofilin phospho (Ser3) antibody WB image

    Western blot: Jurkat cells 1) untreated or 2,3) treated with lambda phosphatase, 3) the phosphatase was inhibited with phospho-Cofilin 1 (Ser3) peptide. The blots were stained with ARG20528 anti-Cofilin phospho (Ser3) antibody.

Specific References

MiR-128-3p suppresses breast cancer cellular progression via targeting LIMK1.

WB / Human

Zhao Junyong et al.
Biomed Pharmacother.,  (2019)

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The microRNAs miR-200b-3p and miR-429-5p target the LIMK1/CFL1 pathway to inhibit growth and motility of breastcancer cells.

WB / Human

Li Deng-Feng et al.
Oncotarget.,  (2017)

publication_link

 

hr_line

MiR-138-5p targeting LIMK1 suppresses breast cancer cell proliferation and motility

WB / Human

Li Deng-Feng et al.
RSC Advances,  (2017)

publication_link

 

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MiR-519d-3p suppresses breast cancer cell growth and motility viatargeting LIM domain kinase 1.

WB / Human

Li Deng-Feng et al.
Mol Cell Biochem.,  (2017)

publication_link

 

hr_line

miR-143-3p targeting LIM domain kinase 1 suppresses the progression of triple-negative breast cancer cells

WB / Human

Dengfeng Li et al.
Am J Transl Res.,  (2017)

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