ARG52247

anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody

anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen I, alpha 1 propeptide (Procollagen I)
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Amph, Bird, Fsh, Mamm
Tested Application IHC-P, WB
Specificity This antibody recognizes pro-collagen I, but not mature form of collagen I.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen I, alpha 1 propeptide (Procollagen I)
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to amino acid residues specific to the collagen I, alpha 1 propeptide.
Conjugation Un-conjugated
Alternate Names OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB1:1,000
Application Note Specific for the propeptide portion of the ~180 kDa collagen I α1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citRate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human lung; Mouse lung; Rat lung

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1277 Human COL1A1

GeneID: 12842 Mouse COL1A1

GeneID: 29393 Rat COL1A1

Gene Symbol COL1A1
Gene Full Name collagen, type I, alpha 1
Background Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).
Highlight Related products:
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Research Area Signaling Transduction antibody
Calculated MW 139 kDa
PTM Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

Images (2) Click the Picture to Zoom In

  • ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody WB image

    Western blot: 30 µg of Rat lung lysate stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody at 1:1000 dilution.

  • ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody IHC image

    Immunohistochemistry: Fibrotic Mouse lung tissue stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody

Application:WB

Sample:Rat lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

Review for anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody

Application:WB

Sample:Mouse lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Xenoextracellular matrix-rosiglitazone complex-mediated immune evasion promotes xenogenic bioengineered root regeneration by altering M1/M2 macrophage polarization

IHC-P / Rat

Tingting Lan et al.
Biomaterials.,  (2021)

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Treated dentin matrix particles combined with dental follicle cell sheet stimulate periodontal regeneration.

WB / Human

Yang Hefeng et al.
Dent Mater.,  (2019)

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