ARG20788

anti-Collagen II antibody (Biotin), pre-adsorbed

anti-Collagen II antibody (Biotin), pre-adsorbed for Electron microscopy,ELISA,FLISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Human,Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Collagen II
Tested Reactivity Hu, Ms, Bov
Tested Application ELISA, EM, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type II collagen. The antibody is pre-adsorbed with Collagen types I, III, IV, V and VI, so the antibody may not react with Collagen types I, III, IV, V and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen II
Antigen Species Human
Immunogen Collagen II
Conjugation Biotin
Full Name collagen, type II, alpha 1
Alternate Names AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen)

Application Instructions

Pre Adsorbed Collagen types I, III, IV, V and VI.
Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:4000
EMAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 142 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.4 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1280 Human COL2A1

GeneID: 12824 Mouse COL2A1

GeneID: 407142 Bovine COL2A1

Gene Symbol COL2A1
Gene Full Name collagen, type II, alpha 1
Background This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt]
PTM Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.