ARG20787

anti-Collagen II antibody, pre-adsorbed

anti-Collagen II antibody, pre-adsorbed for Electron microscopy,ELISA,FLISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Bovine,Chicken,Human,Mouse,Pig,Rabbit,Rat,Sheep

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anti-Collagen II antibody, pre-adsorbed

Goat Polyclonal antibody recognizes Collagen II

ARG20787 Arigo biolaboratories
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anti-Collagen II antibody, pre-adsorbed is rated 4.0 out of 5 by 1.

anti-Collagen II antibody, pre-adsorbed

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Overview

Product Description

Goat Polyclonal antibody recognizes Collagen II

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Pig, Rb, Sheep
Tested Application ELISA, EM, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The antibody reacts with conformational determinants on type II collagen. The antibody is pre-adsorbed with Collagen types I, III, IV, V and VI, so the antibody may not react with Collagen types I, III, IV, V and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen II
Antigen Species Human
Immunogen Collagen II
Conjugation Un-conjugated
Full Name collagen, type II, alpha 1
Alternate Names AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen)

Application Instructions

Pre Adsorbed Collagen types I, III, IV, V and VI.
Application Suggestion
Tested Application Dilution
ELISA1:1,000 – 1:4,000
EMAssay-dependent
FLISA1:200 – 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:100 – 1:300
IPAssay-dependent
WB1:100-1:300
Application Note WB: The antibody reacts with native and denature form of Collagen II protein.
IHC-P: Antigen Retrieval: treat sections with 2 µg/ml proteinase K at 37°C for 15 min before staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 142 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 0.4 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1280 Human COL2A1

GeneID: 12824 Mouse COL2A1

GeneID: 25412 Rat COL2A1

Gene Symbol COL2A1
Gene Full Name collagen, type II, alpha 1
Background This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt]
PTM Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.

Images (4) Click the Picture to Zoom In

  • ARG20787 anti-Collagen II antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Porcine primary chondrocytes fixed with 4% Paraformaldehyde for 10 min at RT and blocked by 1% BSA, 0.1% Triton for 30 min at RT. Cells were incubated in 5% BSA and stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) at 1:100 for 3 hours at RT.

  • ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse rib tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) followed by Rabbit anti-Goat IgG (H+L) antibody (FITC) and DAPI.

  • ARG20787 anti-Collagen II antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded tibiae section from E15 mouse was stained with ARG20787 anti-Collagen II antibody (pre-adsorbed).

  • ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) (right) followed by Donkey anti-Goat IgG (H+L) antibody (Alexa Fluor® 555) and DAPI.

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Application:IF/ICC

Sample:Porcine primary chondrocytes

Fixation Buffer:4% Paraformaldehyde

Fixation Time:10 min

Fixation Temperature:RT ºC

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:3 hours

Primary Antibody Incubation Temperature:RT ºC

Specific References

Shockwave Treatment Enhances the Extracellular Matrix Production in Articular Chondrocyte Through Activation of the ROS/MAPK/Nrf2 Signaling Pathway.

WB / Pig

Shen Po-Chih et al.
Preprint.,  (2020)

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A Decellularized Scaffold Derived From Squid Cranial Cartilage for Use in Cartilage Tissue Engineering.

IHC-P / Squid

Lim Thou et al.
J Mater Chem B.,  (2020)

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A novel elastic and controlled-release poly(ether-ester-urethane)urea scaffold for cartilage regeneration.

IHC-P / Rabbit

Chen Chang'an et al.
J Mater Chem B.,  (2020)

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