ARG20789

anti-Collagen III antibody (Biotin), pre-adsorbed

anti-Collagen III antibody (Biotin), pre-adsorbed for Electron microscopy,ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Dog,Human,Mouse,Rat

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Collagen III
Tested Reactivity Hu, Ms, Rat, Bov, Dog
Tested Application ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type III collagen. The antibody is pre-adsorbed with Collagen types I, II, IV, V and VI, so the antibody may not react with Collagen types I, II, IV, V and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen III
Antigen Species Human
Immunogen Collagen III
Conjugation Biotin
Full Name collagen, type III, alpha 1
Alternate Names EDS4A; Collagen alpha-1(III) chain

Application Instructions

Pre Adsorbed Collagen types I, II, IV, V and VI.
Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:4000
EMAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 139 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.4 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1281 Human COL3A1

GeneID: 12825 Mouse COL3A1

GeneID: 84032 Rat COL3A1

Gene Symbol COL3A1
Gene Full Name collagen, type III, alpha 1
Background This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt]
PTM Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.