ARG20786

anti-Collagen III antibody, pre-adsorbed

anti-Collagen III antibody, pre-adsorbed for ELISA,Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Hamster,Pig,Rabbit

publication_link Publication2

Overview

Product Description

Goat Polyclonal antibody recognizes Collagen III

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dog, Hm, Pig, Rb
Tested Application ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type III collagen. The antibody is pre-adsorbed with Collagen types I, II, IV, V and VI, so the antibody may not react with Collagen types I, II, IV, V and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen III
Species Human
Immunogen Human Type III collagen
Conjugation Un-conjugated
Full Name collagen, type III, alpha 1
Alternate Names EDS4A; Collagen alpha-1(III) chain

Application Instructions

Pre Adsorbed Collagen types I, II, IV, V and VI.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
EMAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1:100 - 1:1000
Application Note WB: The antibody reacts with native and denature form of Collagen III protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 139 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 0.4 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1281 Human COL3A1

GeneID: 12825 Mouse COL3A1

GeneID: 84032 Rat COL3A1

Gene Symbol COL3A1
Gene Full Name collagen, type III, alpha 1
Background This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt]
PTM Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

Images (3) Click the Picture to Zoom In

  • ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG20786 anti-Collagen III antibody, pre-adsorbed WB image

    Western blot: Embryonic chick cardiac tissue samples separated by SDS-PAGE and stained with ARG20786 anti-Collagen III antibody (pre-adsorbed) under reducing conditions.

  • ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

Specific References

Extracorporeal shock wave promotes activation of anterior cruciate ligament remnant cells and their paracrine regulation of bone marrow stromal cells' proliferation, migration, collagen synthesis, and differentiation.

ICC/IF / Human

Cheng-Chang Lu et al.
Bone Joint Res.,  (2020)

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Ba-Wei-Die-Huang-Wan (Hachimi-jio-gan) can ameliorate ketamine-induced cystitis by modulating neuroreceptors, inflammatory mediators, and fibrogenesis in a rat model.

WB / Rat

Lee Wei-Chia et al.
Neurourol Urodyn.,  (2019)

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