anti-Collagen IV alpha 5 antibody [SQab22254]

anti-Collagen IV alpha 5 antibody [SQab22254] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab22254] recognizes Collagen IV alpha 5
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22254
Isotype IgG
Target Name Collagen IV alpha 5
Antigen Species Human
Immunogen Recombinant fragment with COL4A5.
Conjugation Un-conjugated
Protein Full Name Collagen alpha-5(IV) chain
Alternate Names ATS; ASLN; ATS1; CA54; Collagen alpha-5(IV) chain

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human kidney tissue


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1287 Human COL4A5

Swiss-port # P29400 Human Collagen alpha-5(IV) chain

Gene Symbol COL4A5
Gene Full Name collagen type IV alpha 5 chain
Background This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. [UniProt]
Cellular Localization Basement membrane, Extracellular matrix, Secreted. [UniProt]
Calculated MW 161 kDa
PTM Disulfide bond, Glycoprotein, Hydroxylation

Images (1) Click the Picture to Zoom In

  • ARG66926 anti-Collagen IV alpha 5 antibody [SQab22254] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human kidney tissue stained with ARG66926 anti-Collagen IV alpha 5 antibody [SQab22254]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).