anti-Collagen IV antibody

anti-Collagen IV antibody for ICC/IF,Western blot and Human,Mouse

Angiogenesis Study antibody; Basement Membrane Marker antibody


Product Description Rabbit Polyclonal antibody recognizes Collagen IV
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen IV
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 27-54 (N-terminus) of Human Collagen IV alpha 1.
Conjugation Un-conjugated
Alternate Names BSVD; RATOR; Collagen alpha-1(IV) chain

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa


Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1282 Human COL4A1

GeneID: 12826 Mouse COL4A1

Swiss-port # P02462 Human Collagen alpha-1(IV) chain

Swiss-port # P02463 Mouse Collagen alpha-1(IV) chain

Gene Symbol COL4A1
Gene Full Name collagen, type IV, alpha 1
Background Collagen IV proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function Collagen IV is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix, basement membrane
Research Area Angiogenesis Study antibody; Basement Membrane Marker antibody
Calculated MW 161 kDa
PTM Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten.

Images (2) Click the Picture to Zoom In

  • ARG54841 anti-Collagen IV antibody ICC/IF image

    Immunofluorescence: U251 cells stained with ARG54841 anti-Collagen IV antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

  • ARG54841 anti-Collagen IV antibody WB image

    Western blot: 35 µg of HeLa cell lysate stained with ARG54841 anti-Collagen IV antibody at 1:1000 dilution.