anti-Collagen V antibody (Biotin), pre-adsorbed

anti-Collagen V antibody (Biotin), pre-adsorbed for ELISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Electron microscopy,Western blot and Human,Pig,Mouse,Chicken


Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Collagen V
Tested Reactivity Hu, Ms, Chk, Pig
Tested Application ELISA, EM, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type V collagen. The antibody is pre-adsorbed with Collagen types I, II, III, IV and VI, so the antibody may not react with Collagen types I, II, III, IV and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen V
Antigen Species Human
Immunogen Collagen V
Conjugation Biotin
Alternate Names EDSC; Collagen alpha-1(V) chain

Application Instructions

Pre Adsorbed Collagen types I, II, III, IV and VI.
Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:4000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.4 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 12831 Mouse COL5A1

GeneID: 1289 Human COL5A1

Swiss-port # O88207 Mouse Collagen alpha-1(V) chain

Swiss-port # P20908 Human Collagen alpha-1(V) chain

Gene Symbol COL5A1
Gene Full Name collagen, type V, alpha 1
Background This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. [UniProt]
Calculated MW 184 kDa
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.