ARG20790

anti-Collagen V antibody, pre-adsorbed

anti-Collagen V antibody, pre-adsorbed for ELISA,Electron microscopy,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Chicken,Pig

Overview

Product Description Goat Polyclonal antibody recognizes Collagen V
Tested Reactivity Hu, Ms, Chk, Pig
Tested Application ELISA, EM, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type V collagen. The antibody is pre-adsorbed with Collagen types I, II, III, IV and VI, so the antibody may not react with Collagen types I, II, III, IV and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen V
Antigen Species Human
Immunogen Collagen V
Conjugation Un-conjugated
Full Name collagen, type V, alpha 1
Alternate Names EDSC; Collagen alpha-1(V) chain

Application Instructions

Pre Adsorbed Collagen types I, II, III, IV and VI.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
EMAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 184 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 0.4 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12831 Mouse COL5A1

GeneID: 1289 Human COL5A1

Swiss-port # O88207 Mouse Collagen alpha-1(V) chain

Swiss-port # P20908 Human Collagen alpha-1(V) chain

Gene Symbol COL5A1
Gene Full Name collagen, type V, alpha 1
Background This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. [UniProt]
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.

Images (4) Click the Picture to Zoom In

  • ARG20790 anti-Collagen V antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20790 anti-Collagen V antibody (pre-adsorbed) followed by Donkey anti-Goat IgG (H+L) antibody (Alexa Fluor® 555) and DAPI.

  • ARG20790 anti-Collagen V antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20790 anti-Collagen V antibody (pre-adsorbed) followed by Mouse anti-Goat IgG Fc (Alexa Fluor® 555) and DAPI.

  • ARG20790 anti-Collagen V antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20790 anti-Collagen V antibody (pre-adsorbed) followed by Donkey anti-Goat IgG (H+L) antibody (Alexa Fluor® 555) and DAPI.

  • ARG20790 anti-Collagen V antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20790 anti-Collagen V antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.