ARG20793

anti-Collagen VI antibody (Biotin), pre-adsorbed

anti-Collagen VI antibody (Biotin), pre-adsorbed for ELISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Electron microscopy,Western blot and Human,Pig,Mouse,Rat

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Collagen VI
Tested Reactivity Hu, Ms, Rat, Pig
Tested Application ELISA, EM, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type VI collagen. The antibody is pre-adsorbed with Collagen types I, II, III, IV and V, so the antibody may not react with Collagen types I, II, III, IV and V.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen VI
Antigen Species Human
Immunogen Collagen VI
Conjugation Biotin
Full Name collagen, type VI, alpha 1
Alternate Names OPLL; Collagen alpha-1(VI) chain

Application Instructions

Pre Adsorbed Collagen types I, II, III, IV and V.
Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:4000
EMAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 109 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.4 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12833 Mouse COL6A1

GeneID: 1291 Human COL6A1

Swiss-port # P12109 Human Collagen alpha-1(VI) chain

Swiss-port # Q04857 Mouse Collagen alpha-1(VI) chain

Gene Symbol COL6A1
Gene Full Name collagen, type VI, alpha 1
Background The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]
Function Collagen VI acts as a cell-binding protein. [UniProt]
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.