ARG20792

anti-Collagen VI antibody, pre-adsorbed

anti-Collagen VI antibody, pre-adsorbed for ELISA,Electron microscopy,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig

Overview

Product Description Goat Polyclonal antibody recognizes Collagen VI
Tested Reactivity Hu, Ms, Rat, Pig
Tested Application ELISA, EM, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type VI collagen. The antibody is pre-adsorbed with Collagen types I, II, III, IV and V, so the antibody may not react with Collagen types I, II, III, IV and V.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen VI
Antigen Species Human
Immunogen Collagen VI
Conjugation Un-conjugated
Full Name collagen, type VI, alpha 1
Alternate Names OPLL; Collagen alpha-1(VI) chain

Application Instructions

Pre Adsorbed Collagen types I, II, III, IV and V.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
EMAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 109 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 0.4 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12833 Mouse COL6A1

GeneID: 1291 Human COL6A1

Swiss-port # P12109 Human Collagen alpha-1(VI) chain

Swiss-port # Q04857 Mouse Collagen alpha-1(VI) chain

Gene Symbol COL6A1
Gene Full Name collagen, type VI, alpha 1
Background The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]
Function Collagen VI acts as a cell-binding protein. [UniProt]
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Images (2) Click the Picture to Zoom In

  • ARG20792 anti-Collagen VI antibody, pre-adsorbed IHC-Fr image

    Immunohistochemistry: Human kidney cancer tissue stained with ARG20792 anti-Collagen VI antibody (pre-adsorbed) followed by ARG21775 Pig anti-Goat IgG (H+L) antibody (Biotin) (pre-adsorbed) and streptavidin-HRP.

  • ARG20792 anti-Collagen VI antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20792 anti-Collagen VI antibody (pre-adsorbed) (right), followed by ARG21775 Pig anti-Goat IgG (H+L) antibody (Biotin) (pre-adsorbed), ARG23912 Streptavidin (HRP), DAB and hematoxylin.