ARG51827

anti-Cyclin B1 phospho (Ser147) antibody

anti-Cyclin B1 phospho (Ser147) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Cyclin B1 phospho (Ser147)
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cyclin B1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
Conjugation Un-conjugated
Alternate Names G2/mitotic-specific cyclin-B1; CCNB

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 891 Human CCNB1

Swiss-port # P14635 Human G2/mitotic-specific cyclin-B1

Gene Symbol CCNB1
Gene Full Name cyclin B1
Background The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Calculated MW 48 kDa
PTM Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

Images (3) Click the Picture to Zoom In

  • ARG51827 anti-Cyclin B1 phospho (Ser147) antibody WB image

    Western blot: Extracts from MDA cells untreated or treated with Anisomycin stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody.

  • ARG51827 anti-Cyclin B1 phospho (Ser147) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51827 anti-Cyclin B1 phospho (Ser147) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody.