ARG52923

anti-Cyclin D1 antibody [SP4]

anti-Cyclin D1 antibody [SP4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
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Overview

Product Description

Rabbit Monoclonal antibody [SP4] recognizes Cyclin D1

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Dog
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP4
Isotype IgG
Target Name Cyclin D1
Antigen Species Human
Immunogen Synthetic peptide from C-terminus of human cyclin D1.
Conjugation Un-conjugated
Alternate Names B-cell lymphoma 1 protein; PRAD1; U21B31; D11S287E; BCL-1; G1/S-specific cyclin-D1; BCL-1 oncogene; BCL1; PRAD1 oncogene

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-Dependent
IHC-P1:100
WB1:25 - 1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mantle Cell Lymphoma, Tonsil, MCF-7

Properties

Form Liquid
Buffer Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12443 Mouse CCND1

GeneID: 58919 Rat CCND1

GeneID: 595 Human CCND1

Background Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Cellular Localization Nucleus
Highlight Related products:
Cyclin D1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Lymphoma
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 34 kDa
PTM Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization.

Images (13) Click the Picture to Zoom In

  • ARG52923 anti-Cyclin D1 antibody [SP4] WB image

    Western blot: 30 µg of HeLa, SW480, 3T3, and Mouse kidney lysates stained with ARG52923 anti-Cyclin D1 antibody [SP4] at 1:500 dilution.

  • ARG52923 anti-Cyclin D1 antibody [SP4] ICC/IF image

    Immunofluorescence: H1793 cells were fixed with cold methanol:acetone (1:1) for 5 minutes at -20°C, permeabilized with 0.3% Triton X-100 for 30 min at RT then blocked with 1% goat serum for 30 min at RT. Cells were stained with ARG52923 anti-Cyclin D1 antibody [SP4] (red) at 1:100 dilution for 1 hour at RT and DAPI (blue) was used as the nuclear counter stain.

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human B Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Endometrial Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human HK Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Lung Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Ovarian Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Prostate Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Renal Cell Carcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Tonsil stained with Cyclin D1 antibody [SP4] (ARG52923)

  • ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image

    Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)

Customer's Feedback

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Review for anti-Cyclin D1 antibody [SP4]

Application:WB

Sample:Rat kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Multilevel Regulation of β-Catenin Activity by SETD2 Suppresses the Transition from Polycystic Kidney Disease to Clear Cell Renal Cell Carcinoma

WB / Mouse

Hanyu Rao et al.
Cancer Res.,  (2021)

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SETD2 deficiency impairs β-catenin destruction complex to facilitate renal cell carcinoma formation.

WB, IHC-P / Mouse

Hanyu Rao et al.
Preprint.,  (2020)

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ERK Kinases Phosphorylate Lin28a to Modulate P19 Cell Proliferation and Differentiation

WB / Mouse

Xiangyuan Liu et al.
J Biol Chem,  (2017)

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