ARG66804

anti-Cyclin D1 antibody [SQab20217]

anti-Cyclin D1 antibody [SQab20217] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab20217] recognizes Cyclin D1
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab20217
Isotype IgG
Target Name Cyclin D1
Antigen Species Human
Immunogen Synthetic peptide within aa. 200-295 of Human Cyclin D1.
Conjugation Un-conjugated
Alternate Names B-cell lymphoma 1 protein; PRAD1; U21B31; D11S287E; BCL-1; G1/S-specific cyclin-D1; BCL-1 oncogene; BCL1; PRAD1 oncogene

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HCT116; Human colon cancer tissue; Human mantle cell lymphoma
Observed Size ~ 34 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 595 Human CCND1

Swiss-port # P24385 Human G1/S-specific cyclin-D1

Gene Symbol CCND1
Gene Full Name cyclin D1
Background The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019]
Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. [UniProt]
Cellular Localization Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. [UniProt]
Calculated MW 34 kDa
PTM Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.

Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG66804 anti-Cyclin D1 antibody [SQab20217] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66804 anti-Cyclin D1 antibody [SQab20217].

  • ARG66804 anti-Cyclin D1 antibody [SQab20217] WB image

    Western blot: HCT116 cell lysate stained with ARG66804 anti-Cyclin D1 antibody [SQab20217] at 1:1000 dilution.

  • ARG66804 anti-Cyclin D1 antibody [SQab20217] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human mantle cell lymphoma. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66804 anti-Cyclin D1 antibody [SQab20217].

Customer's Feedback

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Review for anti-Cyclin D1 antibody [SQab20217]

Application:WB

Sample:HCT116

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP