anti-Cyclin E1 antibody

anti-Cyclin E1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes Cyclin E1
Tested Reactivity Hu
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cyclin E1
Antigen Species Human
Immunogen Synthetic peptide derived from Human Cyclin E1.
Conjugation Un-conjugated
Alternate Names pCCNE1; CCNE; G1/S-specific cyclin-E1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 50 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 898 Human CCNE1

Swiss-port # P24864 Human G1/S-specific cyclin-E1

Gene Symbol CCNE1
Gene Full Name cyclin E1
Background The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]
Function Essential for the control of the cell cycle at the G1/S (start) transition. [UniProt]
Cellular Localization Nucleus. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Calculated MW 47 kDa
PTM Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.

Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG58045 anti-Cyclin E1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG58045 anti-Cyclin E1 antibody.

Specific References

A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)