ARG54065

anti-Cyclin H antibody

anti-Cyclin H antibody for Western blot,Immunoprecipitation and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody recognizes CCNH
Tested Reactivity Hu
Tested Application IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name Cyclin H
Antigen Species Human
Immunogen Purified recombinant human Cyclin H protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names MO15-associated protein; CAK; CycH; Cyclin-H; p37; p34

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 38 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 902 Human CCNH

Swiss-port # P51946 Human Cyclin-H

Gene Symbol CCNH
Gene Full Name cyclin H
Background Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Function Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. [UniProt]
Cellular Localization Nucleus.
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 38 kDa

Images (2) Click the Picture to Zoom In

  • ARG54065 anti-Cyclin H antibody WB image

    Western blot: A431 cell lysate stained with ARG54065 anti-Cyclin H antibody at 1:1000 dilution.

  • ARG54065 anti-Cyclin H antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54065 anti-Cyclin H antibody.