ARG46716

anti-Cytokeratin 12 antibody [3A3]

anti-Cytokeratin 12 antibody [3A3] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Monoclonal antibody [3A3] recognizes Cytokeratin 12
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone 3A3
Isotype IgG
Target Name Cytokeratin 12
Antigen Species Human
Immunogen Synthetic peptide of human Cytokeratin 12.
Conjugation Un-conjugated
Alternate Names K12; CK-12; Cytokeratin-12; Keratin-12; Keratin, type I cytoskeletal 12

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS;150 mM NaCl, 0.02% sodium azide, 50% glycerol and 0.05% BSA
Preservative 0.02% sodium azide
Stabilizer 50% glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 360625 Rat KRT12

GeneID: 3859 Human KRT12

Swiss-port # Q6IFW5 Rat Keratin, type I cytoskeletal 12

Swiss-port # Q99456 Human Keratin, type I cytoskeletal 12

Gene Symbol KRT12
Gene Full Name keratin 12, type I
Background KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
Function May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity). [UniProt]
Calculated MW 54 kDa

Images (2) Click the Picture to Zoom In

  • ARG46716 anti-Cytokeratin 12 antibody [3A3] WB image

    Western blot: Rat eye stained with ARG46716 anti-Cytokeratin 12 antibody [3A3].

  • ARG46716 anti-Cytokeratin 12 antibody [3A3] WB image

    Western blot: Mouse eye stained with ARG46716 anti-Cytokeratin 12 antibody [3A3].