anti-Cytokeratin 18 antibody [C-04] (FITC)

anti-Cytokeratin 18 antibody [C-04] (FITC) for Flow cytometry and Human,Mouse,Rat,Mammal

Controls and Markers antibody; Signaling Transduction antibody


Product Description FITC-conjugated Mouse Monoclonal antibody [C-04] recognizes Cytokeratin 18
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application FACS
Specificity The clone C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-04
Isotype IgG1
Target Name Cytokeratin 18
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431.
Conjugation FITC
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16668 Mouse KRT18

GeneID: 294853 Rat KRT18

GeneID: 3875 Human KRT18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Images (1) Click the Picture to Zoom In

  • ARG53926 anti-Cytokeratin 18 antibody [C-04] (FITC) FACS image

    Flow Cytometry: HeLa cells stained with ARG53926 anti-Cytokeratin 18 antibody [C-04] (FITC).
    Overlay with Isotype mouse IgG1 control.

Clone References

Luminal cells are favored as the cell of origin for prostate cancer.

Wang ZA et al.
Cell Rep.,  (2014)




TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli.

ICC/IF / Human

Munne PM et al.
Sci Rep.,  (2014)




Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle.

IHC / Mouse

Dowman JK et al.
Am J Pathol.,  (2014)




The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland.

IHC-P / Mouse

Llobet-Navas D et al.
Genes Dev.,  (2014)




Clonogenicity: holoclones and meroclones contain stem cells.

Beaver CM et al.
PLoS One.,  (2014)




Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation.

WB / Mouse

Sanz-Moreno A et al.
PLoS One.,  (2014)




Feasibility of fast dynamic MRI for noninvasive monitoring during ectopic liver cell transplantation to the spleen in a porcine model.

IHC / Pig

Raschzok N et al.
AJR Am J Roentgenol.,  (2012)




Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.

FACS / Human

Chauchereau A et al.
Exp Cell Res.,  (2011)




Suppression of keratin 18 gene expression in bovine blastocysts by RNA interference.

ICC/IF / Cow

Goossens K et al.
Reprod Fertil Dev.,  (2010)