ARG66352

anti-Cytokeratin 18 antibody [SQab18102]

anti-Cytokeratin 18 antibody [SQab18102] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab18102] recognizes Cytokeratin 18
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab18102
Isotype IgG
Target Name Cytokeratin 18
Antigen Species Human
Immunogen Synthetic peptide within aa. 330-430 of Human Cytokeratin 18.
Conjugation Un-conjugated
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HCT116; Human liver tissue
Observed Size ~ 48 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3875 Human KRT18

Swiss-port # P05783 Human Keratin, type I cytoskeletal 18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
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Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.

Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.

O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66352 anti-Cytokeratin 18 antibody [SQab18102] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66352 anti-Cytokeratin 18 antibody [SQab18102] WB image

    Western blot: HCT116 cell lysate stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102] at 1:1000 dilution.

Customer's Feedback

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Review for anti-Cytokeratin 18 antibody [SQab18102]

Application:WB

Sample:HCT116

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP