anti-Cytokeratin 20 antibody

anti-Cytokeratin 20 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

CK7/CK20 Carcinoma Study antibody


Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 20
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 20
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of Human Cytokeratin 20.
Conjugation Un-conjugated
Alternate Names KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Colon Carcinoma


Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 54474 Human KRT20

Swiss-port # P35900 Human Keratin, type I cytoskeletal 20

Background Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt]
Cellular Localization Cytoplasm
Research Area CK7/CK20 Carcinoma Study antibody
Calculated MW 48 kDa
PTM Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

Images (1) Click the Picture to Zoom In

  • ARG53194 anti-Cytokeratin 20 antibody IHC-P image

    Immunohistochemistry: Human Colon Adenocarcinoma stained with ARG53194 anti-Cytokeratin 20 antibody.