anti-Cytokeratin 20 antibody [SQab1737]
anti-Cytokeratin 20 antibody [SQab1737] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
CK7/CK20 Carcinoma Study antibody
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1737] recognizes Cytokeratin 20|
|Tested Application||FACS, ICC/IF, IHC-P, IP, WB|
|Target Name||Cytokeratin 20|
|Immunogen||Synthetic peptide around the C-terminus of Human Cytokeratin 20.|
|Alternate Names||KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||keratin 20, type I|
|Background||Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]|
|Function||Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt]|
|Highlight||Related Antibody Duos and Panels:
ARG30318 CK7 / CK20 Carcinoma Antibody Duo
Cytokeratin 20 antibodies; Cytokeratin 20 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer
|Research Area||CK7/CK20 Carcinoma Study antibody|
|Calculated MW||48 kDa|
|PTM||Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. [UniProt]
Images (5) Click the Picture to Zoom In
ARG66248 anti-Cytokeratin 20 antibody [SQab1737] ICC/IF image
Immunofluorescence: HT-29 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at RT. Samples were stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (green) at 1:200, 4°C.
ARG66248 anti-Cytokeratin 20 antibody [SQab1737] FACS image
Flow Cytometry: HT-29 cells stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the primary antibody at 1:1000 dilution in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat anti-rabbit Alexa Fluor® 488 (IgG H+L) at 1:2000 dilution for 20 min at 4°C. Unlabelled sample (Blue) was used as a control.
ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded colonic adenocarcinoma stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:800. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
ARG66248 anti-Cytokeratin 20 antibody [SQab1737] WB image
Western blot: 10 μg of HCT-116 and HT-29 cell lysates stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:5000 dilution.
ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IP image
Immunoprecipitation: Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:50 dilution.
1. IP by using ARG66248 in HT-29 whole cell lysate
2. PBS instead of ARG66248 in HT-29 whole cell lysate
3. 10 µg of HT-29 whole cell lysate (input)