anti-Cytokeratin 6 antibody [SQab18112]

anti-Cytokeratin 6 antibody [SQab18112] for ICC/IF and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab18112] recognizes Cytokeratin 6
Tested Reactivity Hu
Tested Application ICC/IF
Host Rabbit
Clonality Monoclonal
Clone SQab18112
Isotype IgG
Target Name Cytokeratin 6
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human Cytokeratin 6.
Conjugation Un-conjugated
Alternate Names CK-6A; K6D; Cytokeratin-6A; CK-6D; K6C; K6A; Type-II keratin Kb6; PC3; CK6D; KRT6D; CK6A; CK6C; allergen Hom s 5; Keratin, type II cytoskeletal 6A; Keratin-6A; Cytokeratin-6D; KRT6C

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3853 Human KRT6A

Swiss-port # P02538 Human Keratin, type II cytoskeletal 6A

Gene Symbol KRT6A
Gene Full Name keratin 6A, type II
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]
Function Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. [UniProt]
Calculated MW 60 kDa

Images (1) Click the Picture to Zoom In

  • ARG66385 anti-Cytokeratin 6 antibody [SQab18112] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Samples were stained with ARG66385 anti-Cytokeratin 6 antibody [SQab18112] (1:50) at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.