ARG20785

anti-Cytokeratin 7 antibody [RCK105]

anti-Cytokeratin 7 antibody [RCK105] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat

CK7/CK20 Carcinoma Study antibody

Overview

Product Description

Mouse Monoclonal antibody [RCK105] recognizes Cytokeratin 7

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cat, Dog, Hm, Pig
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone RCK105
Isotype IgG1
Target Name Cytokeratin 7
Antigen Species Human
Immunogen A cytokeratin from Human bladder carcinoma cell line T24 and SP2/0-Ag14 Mouse myeloma cells.
Conjugation Un-conjugated
Full Name keratin 7, type II
Alternate Names SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:200
ICC/IF1:100 - 1:200
IHC-Fr1:100 - 1:200
IHC-PAssay-dependent
WB1:100 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 51 kDa

Properties

Form Liquid
Purification Purification with Protein A/G.
Buffer PBS and 0.08% Sodium azide
Preservative 0.08% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 110310 Mouse KRT7

GeneID: 300242 Rat KRT7

GeneID: 3855 Human KRT7

Gene Symbol KRT7
Gene Full Name keratin 7, type II
Background Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Function Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt]
Research Area CK7/CK20 Carcinoma Study antibody
PTM Arg-20 is dimethylated, probably to asymmetric dimethylarginine.