ARG66340

anti-Cytokeratin 7 antibody [SQab1888]

anti-Cytokeratin 7 antibody [SQab1888] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF,Immunoprecipitation and Human,Mouse

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anti-Cytokeratin 7 antibody [SQab1888] Recombinant Rabbit Monoclonal antibody [SQab1888] recognizes Cytokeratin 7 ARG66340 Arigo biolaboratories
5.0 out of 5 based on 2
anti-Cytokeratin 7 antibody [SQab1888] is rated 5.0 out of 5 by 2.

anti-Cytokeratin 7 antibody [SQab1888]

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1888] recognizes Cytokeratin 7
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1888
Isotype IgG
Target Name Cytokeratin 7
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 1-100 of Cytokeratin 7.
Conjugation Un-conjugated
Full Name keratin 7, type II
Alternate Names SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:800
ICC/IF1:800 - 1:2000
IHC-P1:1600 - 1:3200
IP1:50
WB1:2000 - 1:5000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 51 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 110310 Mouse KRT7

GeneID: 3855 Human KRT7

Swiss-port # P08729 Human Keratin, type II cytoskeletal 7

Swiss-port # Q9DCV7 Mouse Keratin, type II cytoskeletal 7

Gene Symbol KRT7
Gene Full Name keratin 7, type II
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt]
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PTM Arg-20 is dimethylated, probably to asymmetric dimethylarginine. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG66340 anti-Cytokeratin 7 antibody [SQab1888] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (green) at 1:2,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66340 anti-Cytokeratin 7 antibody [SQab1888] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human thyroid cancer tissue stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:3,200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66340 anti-Cytokeratin 7 antibody [SQab1888] WB image

    Western blot: 10 µg of HeLa, A549 and HaCat cell lysates stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:10000 dilution.

  • ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IP image

    Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888]. 1) ARG66340 IP in HeLa whole cell lysate, 2) PBS instead of ARG66340 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).

Customer's Feedback

nuts_pic      Excellent

Review for anti-Cytokeratin 7 antibody [SQab1888]

Application:IHC

Sample:Mouse tumor

Sample Preparation Method:Paraffin-embedded

Fixation Buffer:10% Formalin

Fixation Time:

Fixation Temperature: ºC

Antigen Retrieval Buffer:Tris/EDTA buffer (pH 9.0)

show all review

nuts_pic      Excellent

Review for anti-Cytokeratin 7 antibody [SQab1888]

Application:IHC

Sample:Mouse tumor

Sample Preparation Method:Paraffin-embedded

Fixation Buffer:10% Formalin

Fixation Time:

Fixation Temperature: ºC

Antigen Retrieval Buffer:Tris/EDTA buffer (pH 9.0)

show all review