anti-Cytokeratin 8 + 18 antibody [SQab20229]

anti-Cytokeratin 8 + 18 antibody [SQab20229] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab20229] recognizes Cytokeratin 8 + 18
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab20229
Isotype IgG
Target Name Cytokeratin 8 + 18
Antigen Species Human
Immunogen Synthetic peptide within aa. 383 to C-terminal of Human Cytokeratin 8 and synthetic peptide within aa. 330-430 of Human Cytokeratin 18.
Conjugation Un-conjugated
Alternate Names KRT8: Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8
KRT18: Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Gene Symbol KRT8; KRT18
Gene Full Name keratin 8, type II; keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Cellular Localization KRT8: Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. [UniProt]
Calculated MW 54 kDa
PTM KRT8: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.

O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt]

KRT18: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.

Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.

O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229].