anti-Cytokeratin (pan) antibody [C-11] (FITC)

anti-Cytokeratin (pan) antibody [C-11] (FITC) for Flow cytometry and Human,Mouse,Rat,Mammal

Controls and Markers antibody; Signaling Transduction antibody


Product Description FITC-conjugated Mouse Monoclonal antibody [C-11] recognizes Cytokeratin (pan)
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application FACS
Specificity The clone C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-11
Isotype IgG1
Target Name Cytokeratin (pan)
Antigen Species Human
Immunogen Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Conjugation FITC
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16668 Mouse KRT18

GeneID: 294853 Rat KRT18

GeneID: 3875 Human KRT18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Images (1) Click the Picture to Zoom In

  • ARG54232 anti-Cytokeratin (pan) antibody [C-11] (FITC) FACS image

    Flow Cytometry: Separation of HeLa cells (red-filled) from human peripheral whole blood (black-dashed). Cells were stained with ARG54232 anti-Cytokeratin (pan) antibody [C-11] (FITC) at 3 µg/ml dilution.