anti-Cytokeratin (pan) antibody [PAN-CK]

anti-Cytokeratin (pan) antibody [PAN-CK] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

publication_link Publication1


Product Description Mouse Monoclonal antibody [PAN-CK] recognizes Cytokeratin (pan)
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P
Specificity Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK7, CK8, CK10, CK13, CK14, CK15, CK16, CK17, CK18 and CK19.
Host Mouse
Clonality Monoclonal
Clone PAN-CK
Isotype IgG, kappa
Target Name Cytokeratin (pan)
Antigen Species Human
Immunogen Human epidermal keratin
Conjugation Un-conjugated
Alternate Names Type-II keratin Kb39; KRT1B; Keratin-77; K1B; K77; CK-1B; Cytokeratin-1B; Keratin, type II cytoskeletal 1b

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 374454 Human KRT77

GeneID: 406220 Mouse KRT77

GeneID: 406226 Rat KRT77

Gene Symbol KRT77
Gene Full Name keratin 77, type II
Background Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
Calculated MW 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 55 kDa (CK7); 52 kDa (CK8); 56.5 kDa (CK10); 53 kDa (CK13); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 46 kDa (CK17); 45 kDa (CK18) and 40 kDa (CK19).
PTM Undergoes deimination of some arginine residues (citrullination).

Images (1) Click the Picture to Zoom In

  • ARG56128 anti-Cytokeratin (pan) antibody [PAN-CK] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human skin stained with ARG56128 anti-Cytokeratin (pan) antibody [PAN-CK].

Specific References

Long-term remission under Disitamab Vedotin (RC48) in HR-positive/HER2-positive metastatic breast cancer with brain meningeal, and bone marrow involvement: A case report

IHC-P / Human

Qifeng Wu et al.
Oncol Lett.,  (2022)