ARG10354

anti-D-Dimer antibody [DD1]

anti-D-Dimer antibody [DD1] for ELISA,Immunoassay,Western blot and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody [DD1] recognizes D-Dimer
Tested Reactivity Hu
Tested Application ELISA, IA, WB
Specificity Do not cross-react with fibrinogen.
Host Mouse
Clonality Monoclonal
Clone DD1
Isotype IgG2a
Target Name D-Dimer
Antigen Species Mouse
Immunogen homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Conjugation Un-conjugated

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Resrarch Area Cell Biology and Cellular Response antibody

Images (1) Click the Picture to Zoom In

  • ARG10354 anti-D-Dimer antibody [DD1] WB image

    Western Blot: D-dimer was run in SDS-PAGE under non-reducing (A) or reducing (B) conditions. Using a 7.5–12.5% separating gel and transferred onto a nitrocellulose membrane.

    The membrane was blocked by 7% milk in PBST for 30 minutes and the protein bands were stained by different 4 D-dimer mAbs (10 µg/ml) 1) anti-D-Dimer antibody [DD1] (ARG10354); 2) anti-D-Dimer antibody [DD189]; 3) anti-D-Dimer antibody [DD255] stained with anti-D-Dimer antibody [DD1] (ARG10354) for 1 hour. After washing with PBST, goat anti-mouse Fc-specific IgG labeled with horseradish peroxidase was added and incubated for 1 hour. After washing with PBST, the immune complexes were visualized by DAB/hydrogen peroxide in 50 mM Tris-HCl buffer, pH7.5.

Clone References

Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring.

ELISA / Human

Anderson N et al.
Int J Colorectal Dis.,  (2011)

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Activation of mannan-binding lectin-associated serine proteases leads to generation of a fibrin clot.

Gulla KC et al.
Immunology.,  (2010)

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Activation of plasminogen activator inhibitor implicates protease InhA in the acute-phase response to Bacillus anthracis infection.

ELISA / 

Chung MC et al.
J Med Microbiol.,  (2009)

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Neutrophil elastase and syndecan shedding contribute to antithrombin depletion in murine anthrax.

Chung MC et al.
FEMS Immunol Med Microbiol.,  (2008)

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Free antibody sample