ARG52254

anti-DARPP32 antibody

anti-DARPP32 antibody for Western blot,Immunohistochemistry,ICC/IF and Rat

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DARPP32
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis
Tested Application ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DARPP32
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Conjugation Un-conjugated
Alternate Names Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC1:250
WB1:1,000
Application Note Specific for the ~32k DARPP-32 protein
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 360616 Rat PPP1R1B

Swiss-port # Q6J4I0 Rat Protein phosphatase 1 regulatory subunit 1B

Gene Symbol PPP1R1B
Gene Full Name protein phosphatase 1, regulatory (inhibitor) subunit 1B
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002)
Research Area Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 23 kDa
PTM Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.

Images (1) Click the Picture to Zoom In

  • ARG52254 anti-DARPP32 antibody WB image

    Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein stained with ARG52254 anti-DARPP32 antibody.