anti-DARPP32 phospho (Thr75) antibody
anti-DARPP32 phospho (Thr75) antibody for Western blot and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes DARPP32 phospho (Thr75)|
|Predict Reactivity||Hu, Ms, Bov, Chk, Dog, NHuPrm|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH|
|Alternate Names||Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B|
|Application Note||Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by λ-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||protein phosphatase 1, regulatory (inhibitor) subunit 1B|
|Background||DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).|
|Research Area||Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody|
|Calculated MW||23 kDa|
|PTM||Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
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