ARG54843

anti-DCLK1 antibody

anti-DCLK1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Neuroscience antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes DCLK1

Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DCLK1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 690-720 (C-terminus) of Human DCLK1.
Conjugation Un-conjugated
Alternate Names DCAMKL1; DCLK; Serine/threonine-protein kinase DCLK1; Doublecortin-like and CAM kinase-like 1; DCDC3A; EC 2.7.11.1; Doublecortin domain-containing protein 3A; CL1; Doublecortin-like kinase 1; CLICK1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13175 Mouse DCLK1

GeneID: 9201 Human DCLK1

Swiss-port # O15075 Human Serine/threonine-protein kinase DCLK1

Swiss-port # Q9JLM8 Mouse Serine/threonine-protein kinase DCLK1

Gene Symbol DCLK1
Gene Full Name doublecortin-like kinase 1
Background This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
Function Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. [UniProt]
Research Area Neuroscience antibody
Calculated MW 82 kDa

Images (2) Click the Picture to Zoom In

  • ARG54843 anti-DCLK1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG54843 anti-DCLK1 antibody.

  • ARG54843 anti-DCLK1 antibody WB image

    Western blot: 35 µg of Mouse heart lysate stained with ARG54843 anti-DCLK1 antibody.