ARG45478
anti-DDAH1 antibody
anti-DDAH1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DDAH1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDAH1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human DDAH1. |
| Conjugation | Un-conjugated |
| Alternate Names | Dimethylarginine dimethylaminohydrolase 1; DDAHI; G; DDAH; EC 3.5.3.18; N; Dimethylargininase-1; HEL-S-16; DDAH-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 31 kDa, 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DDAH1 |
| Gene Full Name | Dimethylarginine Dimethylaminohydrolase 1 |
| Background | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
| Function | Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | Acetylation; S-nitrosylation. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG45478 anti-DDAH1 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG45478 anti-DDAH1 antibody at 2 μg/ml dilution.
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ARG45478 anti-DDAH1 antibody WB image
Western blot: HepG2 stained with ARG45478 anti-DDAH1 antibody at 0.5 μg/ml dilution.
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ARG45478 anti-DDAH1 antibody FACS image
Flow Cytometry: A431 stained with ARG45478 anti-DDAH1 antibody at 1 µg/10^6 cells dilution.
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ARG45478 anti-DDAH1 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45478 anti-DDAH1 antibody at 1 μg/ml dilution.
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ARG45478 anti-DDAH1 antibody WB image
Western blot: Rat brain stained with ARG45478 anti-DDAH1 antibody at 0.5 μg/ml dilution.
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ARG45478 anti-DDAH1 antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45478 anti-DDAH1 antibody at 1 μg/ml dilution.
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ARG45478 anti-DDAH1 antibody WB image
Western blot: Mouse brain stained with ARG45478 anti-DDAH1 antibody at 0.5 μg/ml dilution.
