ARG66332

anti-DDDDK tag antibody [SQab1880]

anti-DDDDK tag antibody [SQab1880] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1880] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
The antibody recognizes the hydrophilic epitope tagging peptide sequence DDDDK (Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1880
Isotype IgG
Target Name DDDDK tag
Antigen Species Others
Immunogen KLH-conjugated DYKDDDDK.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:1000 - 1:2000
ICC/IF1:1500 - 1:10000
IP1:50
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (5) Click the Picture to Zoom In

  • ARG66332 anti-DDDDK tag antibody [SQab1880] ICC/IF image

    Immunofluorescence: 293 cells trasnfected with C-terminal DYKDDDDK tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with DAPI (left) or ARG66332 anti-DDDDK tag antibody [SQab1880] at 1:10000 and 4°C (right).

  • ARG66332 anti-DDDDK tag antibody [SQab1880] FACS image

    Flow Cytometry: 293 cells transfected with N-terminal DYKDDDDK tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66332 anti-DDDDK tag antibody [SQab1880] (light blue histogram) at 1:2000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (white histogram) was used as a control.

  • ARG66332 anti-DDDDK tag antibody [SQab1880] WB image

    Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 µg of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with N-terminal DYKDDDDK tagged gene (ARG66332 at 1:10000, right).

  • ARG66332 anti-DDDDK tag antibody [SQab1880] IP image

    Immunoprecipitation: 0.1 mg of 293 whole cells lysate transfected with N-terminal His tagged gene immunoprecipitated (1:50) and stained with ARG66332 anti-DDDDK tag antibody [SQab1880]. 1) PBS (Control) and 2) Primary antibody.

  • ARG66332 anti-DDDDK tag antibody [SQab1880] WB image

    Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 µg of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with C-terminal DYKDDDDK tagged gene (ARG66332 at 1:100000, right).