ARG46118
anti-DGCR8 antibody
anti-DGCR8 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DGCR8 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DGCR8 |
| Antigen Species | Human |
| Immunogen | A 14 amino acid synthetic peptide within aa. 390 - 440 of human DGCR8. |
| Conjugation | Un-conjugated |
| Alternate Names | DDC; DOPA decarboxylase; AADC; DDC; Aromatic-L-amino-acid decarboxylase; EC 4.1.1.28 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 53 kDa |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DDC |
| Gene Full Name | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| Background | The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010). |
| Calculated MW | 53 kDa |
Images (2) Click the Picture to Zoom In
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ARG46118 anti-DGCR8 antibody IHC-P image
Immunohistochemistry: Human lung stained with ARG46118 anti-DGCR8 antibody.
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ARG46118 anti-DGCR8 antibody WB image
Western blot: Human lung stained with ARG46118 anti-DGCR8 antibody.
