anti-DGCR8 phospho (Ser377) antibody
anti-DGCR8 phospho (Ser377) antibody for Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes DGCR8 phospho (Ser377)|
|Tested Reactivity||Hu, Ms|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH|
|Alternate Names||DOPA decarboxylase; AADC; DDC; Aromatic-L-amino-acid decarboxylase; EC 22.214.171.124|
|Application Note||Specific for the ~120k DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||dopa decarboxylase (aromatic L-amino acid decarboxylase)|
|Background||The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).|
|Research Area||Cancer antibody; Metabolism antibody; Neuroscience antibody|
|Calculated MW||54 kDa|
Images (1) Click the Picture to Zoom In
ARG52258 anti-DGCR8 phospho (Ser377) antibody WB image
Western blot: Mouse nuclei lysate showing specific immunolabeling of the ~120k DGCR8 protein phosphorylated at Ser 377 (control) by using ARG52258 anti-DGCR8 phospho (Ser377) antibody.
The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos pep).