ARG52258

anti-DGCR8 phospho (Ser377) antibody

anti-DGCR8 phospho (Ser377) antibody for Western blot and Human,Mouse

Cancer antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DGCR8 phospho (Ser377)
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DGCR8
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH
Conjugation Un-conjugated
Alternate Names DOPA decarboxylase; AADC; DDC; Aromatic-L-amino-acid decarboxylase; EC 4.1.1.28

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1,000
Application Note Specific for the ~120k DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13195 Mouse DDC

GeneID: 1644 Human DDC

Swiss-port # O88533 Mouse Aromatic-L-amino-acid decarboxylase

Swiss-port # P20711 Human Aromatic-L-amino-acid decarboxylase

Gene Symbol DGCR8
Gene Full Name dopa decarboxylase (aromatic L-amino acid decarboxylase)
Background The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 54 kDa

Images (1) Click the Picture to Zoom In

  • ARG52258 anti-DGCR8 phospho (Ser377) antibody WB image

    Western blot: Mouse nuclei lysate showing specific immunolabeling of the ~120k DGCR8 protein phosphorylated at Ser 377 (control) by using ARG52258 anti-DGCR8 phospho (Ser377) antibody.
    The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos pep).