ARG45513
anti-DMAP1 antibody
anti-DMAP1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DMAP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DMAP1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DMAP1. |
| Conjugation | Un-conjugated |
| Alternate Names | DMAP1; DNA Methyltransferase 1 Associated Protein 1; DNMAP1; KIAA1425; DNMTAP1; MEAF2; EAF2; SWC4; DNA Methyltransferase 1-Associated Protein 1; FLJ11543; DNMT1 Associated Protein 1; DNMT1-Associated Protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 53 - 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9JI44 Mouse DNA methyltransferase 1-associated protein 1 Swiss-port # Q9NPF5 Human DNA methyltransferase 1-associated protein 1 |
|---|---|
| Gene Symbol | DMAP1 |
| Gene Full Name | DNA Methyltransferase 1 Associated Protein 1 |
| Background | This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| Function | Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45513 anti-DMAP1 antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45513 anti-DMAP1 antibody at 5 μg/ml dilution.
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ARG45513 anti-DMAP1 antibody WB image
Western blot: MCF-7 stained with ARG45513 anti-DMAP1 antibody at 0.5 μg/ml dilution.
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ARG45513 anti-DMAP1 antibody FACS image
Flow Cytometry: CACO-2 stained with ARG45513 anti-DMAP1 antibody at 1 µg/10^6 cells dilution.
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ARG45513 anti-DMAP1 antibody WB image
Western blot: Rat kidney stained with ARG45513 anti-DMAP1 antibody at 0.5 μg/ml dilution.
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ARG45513 anti-DMAP1 antibody WB image
Western blot: NIH/3T3 stained with ARG45513 anti-DMAP1 antibody at 0.5 μg/ml dilution.
