ARG10650

anti-DMPK antibody

anti-DMPK antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes DMPK
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Gpig, Hrs
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DMPK
Antigen Species Human
Immunogen Synthetic peptide around the center region of Human DMPK.
Conjugation Un-conjugated
Full Name dystrophia myotonica-protein kinase
Alternate Names Myotonin-protein kinase; DM; MDPK; DM1 protein kinase; MT-PK; DMK; DM-kinase; DM1; DMPK; EC 2.7.11.1; Myotonic dystrophy protein kinase; DM1PK

Application Instructions

Predict Reactivity Note Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rat: 100%
Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.2 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 69 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.09% (w/v) Sodium azide and 2% Sucrose.
Preservative 0.09% (w/v) Sodium azide
Stabilizer 2% Sucrose
Concentration 0.5 - 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1760 Human DMPK

Swiss-port # Q09013 Human Myotonin-protein kinase

Gene Symbol DMPK
Gene Full Name dystrophia myotonica-protein kinase
Background The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Function Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity. [UniProt]
PTM Phosphorylated. Autophosphorylates. Phosphorylation by RAF1 may result in activation of DMPK.
Proteolytic processing of the C-terminus may remove the transmembrane domain and release the kinase from membranes stimulating its activity.

Images (2) Click the Picture to Zoom In

  • ARG10650 anti-DMPK antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG10650 anti-DMPK antibody at 5 µg/ml dilution.

  • ARG10650 anti-DMPK antibody WB image

    Western blot: Human fetal muscle lysate stained with ARG10650 anti-DMPK antibody at 1 µg/ml dilution.