anti-DMPO Nitrone Adduct antibody [N166A4]

anti-DMPO Nitrone Adduct antibody [N166A4] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,ELISA and Other

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody [N1664A] recognizes DMPO Nitrone Adduct
Tested Reactivity Other
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Host Mouse
Clonality Monoclonal
Clone N1664A
Isotype IgG1
Target Name DMPO Nitrone Adduct
Immunogen 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
IP25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Background The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Images (2) Click the Picture to Zoom In

  • ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] ICC/IF image

    Immunofluorescence: Mouse macrophage cell lines stained with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] at 10 ug/ml dilution.

  • ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] WB image

    Western blot: 1) 10 uM Hb (Hemoglobin), 2) 10 uM Hb + 100 uM HOCI, 3) 10 uM Hb + 500 uM HOCI, 4) 10 uM Hb + 1000 uM HOCI, 5)-8) as above with 20 mM DMPO, 9)-12) as above with 100 mM DMPO. Staining with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4].