ARG45722
anti-DNAJC6 antibody
anti-DNAJC6 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNAJC6 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNAJC6 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DNAJC6. |
| Conjugation | Un-conjugated |
| Alternate Names | DNAJC6; DnaJ Heat Shock Protein Family (Hsp40) Member C6; KIAA0473; Auxilin; PARK19; DnaJ (Hsp40) Homolog, Subfamily C, Member 6; Putative Tyrosine-Protein Phosphatase Auxilin; DnaJ (Hsp40) Homolog, Subfamily B, Member 6; DnaJ Homolog Subfamily C Member 6; EC 3.1.3.-; DJC6 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 120 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75061 Human Putative tyrosine-protein phosphatase auxilin Swiss-port # Q80TZ3 Mouse Putative tyrosine-protein phosphatase auxilin |
|---|---|
| Gene Symbol | DNAJC6 |
| Gene Full Name | DnaJ Heat Shock Protein Family (Hsp40) Member C6 |
| Background | DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008] |
| Function | May act as a protein phosphatase and/or a lipid phosphatase. Co-chaperone that recruits HSPA8/HSC70 to clathrin-coated vesicles (CCVs) and promotes the ATP-dependent dissociation of clathrin from CCVs and participates in clathrin-mediated endocytosis of synaptic vesicles and their recycling and also in intracellular trafficking. [UniProt] |
| Cellular Localization | Cytoplasmic vesicle. [UniProt] |
| Calculated MW | 100 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
-
ARG45722 anti-DNAJC6 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45722 anti-DNAJC6 antibody at 5 μg/ml dilution.
-
ARG45722 anti-DNAJC6 antibody WB image
Western blot: HEL stained with ARG45722 anti-DNAJC6 antibody at 0.5 μg/ml dilution.
-
ARG45722 anti-DNAJC6 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45722 anti-DNAJC6 antibody at 2 μg/ml dilution.
-
ARG45722 anti-DNAJC6 antibody WB image
Western blot: PC-12 stained with ARG45722 anti-DNAJC6 antibody at 0.5 μg/ml dilution.
-
ARG45722 anti-DNAJC6 antibody WB image
Western blot: Mouse brain stained with ARG45722 anti-DNAJC6 antibody at 0.5 μg/ml dilution.
