ARG54335

anti-DNase II antibody

anti-DNase II antibody for Western blot,Immunohistochemistry and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DNase II
Tested Reactivity Hu
Tested Application IHC, WB
Specificity This antibody recognizes the proenzyme of human DNase II (40kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DNase II
Antigen Species Human
Immunogen Peptide corresponding to aa 347-360 of human DNase II precursor (accession no. AF047016).
Conjugation Un-conjugated
Alternate Names EC 3.1.22.1; DNASE2A; Deoxyribonuclease II alpha; DNL2; DNL; Deoxyribonuclease-2-alpha; Acid DNase; R31240_2; Lysosomal DNase II; DNase II alpha

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human spleen

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1777 Human DNASE2

Swiss-port # O00115 Human Deoxyribonuclease-2-alpha

Gene Symbol DNASE2
Gene Full Name deoxyribonuclease II, lysosomal
Background DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa and 12 kDa chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
Function Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 40 kDa
PTM Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.

Images (2) Click the Picture to Zoom In

  • ARG54335 anti-DNase II antibody WB image

    Western Blot: human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide stained with ARG54335 anti-DNase II antibody at 1 μg/ml dilution.

  • ARG54335 anti-DNase II antibody IHC image

    Immunohistochemistry: human spleen stained with ARG54335 anti-DNase II antibody at 5 μg/ml dilution.