ARG45212
anti-DPH5 antibody
anti-DPH5 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DPH5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DPH5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DPH5. |
| Conjugation | Un-conjugated |
| Alternate Names | DPH5; Diphthamide Biosynthesis 5; Diphthine Methyl Ester Synthase; CGI-30; Diphthamide Biosynthesis Methyltransferase; DPH5 Homolog (S. Cerevisiae); Diphthine Synthase; Protein X 0011; DPH5 Homolog; EC 2.1.1.314; EC 2.1.1.98; HSPC143; AD-018; NEDSFF; NPD015 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DPH5 |
| Gene Full Name | Diphthamide Biosynthesis 5 |
| Background | Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.. [UniProt] |
| Cellular Localization | cytosol. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
-
ARG45212 anti-DPH5 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45212 anti-DPH5 antibody at 5 ug/ml dilution.
-
ARG45212 anti-DPH5 antibody WB image
Western blot: 293T, HepG2, K562, and Daudi stained with ARG45212 anti-DPH5 antibody at 0.5 μg/ml dilution.
-
ARG45212 anti-DPH5 antibody FACS image
Flow Cytometry: A549 stained with ARG45212 anti-DPH5 antibody at 1 µg/10^6 cells dilution.
