ARG65119
anti-DTX3 antibody
anti-DTX3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DTX3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DTX3 |
| Antigen Species | Human |
| Immunogen | C-KNKVTVSKPVWD |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.3.2.-; RING finger protein 154; RNF154; Deltex3; deltex3; Probable E3 ubiquitin-protein ligase DTX3; Protein deltex-3 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80V91 Mouse Probable E3 ubiquitin-protein ligase DTX3 Swiss-port # Q8N9I9 Human Probable E3 ubiquitin-protein ligase DTX3 |
|---|---|
| Background | DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] |
| Research Area | Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
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ARG65119 anti-DTX3 antibody WB image
Western Blot: NIH3T3 lysate (35 µg protein in RIPA buffer) stained with ARG65119 anti-DTX3 antibody at 0.3 µg/ml dilution.
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ARG65119 anti-DTX3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65119 anti-DTX3 antibody at 10 µg/ml dilution followed by AP-staining.
