ARG20542

anti-DUX4 antibody [P2B1]

anti-DUX4 antibody [P2B1] for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse

Developmental Biology antibody

Overview

Product Description Mouse Monoclonal antibody [P2B1] recognizes DUX4
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC, WB
Specificity This antibody recognizes human DUX4. Iut does not cross react with DUX4c.
Host Mouse
Clonality Monoclonal
Clone P2B1
Isotype IgG1
Target Name DUX4
Species Human
Immunogen Synthetic peptide around 76 aa (C-terminus) of Human DUX4 with glutathione-s-transferase (gst) tag.
Conjugation Un-conjugated
Full Name double homeobox 4 like 1
Alternate Names DUX4; DUX10

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHCAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 45 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol DUX4L1
Gene Full Name double homeobox 4 like 1
Background DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1. Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues.
Cellular Localization Nuclear
Research Area Developmental Biology antibody

Images (2) Click the Picture to Zoom In

  • ARG20542 anti-DUX4 antibody [P2B1] ICC/IF image

    Immunofluorescence: C2C12 myoblasts transfected with pCS2+DUX4, stained with ARG20542 anti-DUX4 antibody [P2B1].  Counter-stained with DAPI for nuclei.

  • ARG20542 anti-DUX4 antibody [P2B1] WB image

    Western blot: C2C12 cells transfected with pCS2+DUX4 which contains an additional upstream start site, stained with ARG20542 anti-DUX4 antibody [P2B1].