ARG66200

anti-Desmin antibody [SQab1722]

anti-Desmin antibody [SQab1722] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
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anti-Desmin antibody [SQab1722]

Recombinant Rabbit Monoclonal antibody [SQab1722] recognizes Desmin

ARG66200 Arigo biolaboratories
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anti-Desmin antibody [SQab1722] is rated 5.0 out of 5 by 1.

anti-Desmin antibody [SQab1722]

Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1722] recognizes Desmin

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1722
Isotype IgG
Target Name Desmin
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Desmin.
Conjugation Un-conjugated
Full Name desmin
Alternate Names LGMD2R; CSM1; Desmin; CSM2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:4000 ‐ 1:8000
WB1:5000 ‐ 1:10000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13346 Mouse DES

GeneID: 1674 Human DES

GeneID: 64362 Rat DES

Gene Symbol DES
Gene Full Name desmin
Background Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Function Desmin: Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30327 Microvascular Density Antibody Panel
Related products:
Desmin antibodies; Desmin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
PTM ADP-ribosylation prevents ability to form intermediate filaments.

Images (3) Click the Picture to Zoom In

  • ARG66200 anti-Desmin antibody [SQab1722] WB image

    Western blot: 30 µg of MEF (negative control) and Mouse heart lysates stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:5000 dilution.

  • ARG66200 anti-Desmin antibody [SQab1722] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:8000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66200 anti-Desmin antibody [SQab1722] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human placenta tissue stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:8000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

Customer's Feedback

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Review for anti-Desmin antibody [SQab1722]

Application:WB

Sample:Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC