ARG58558

anti-Desmoglein 3 antibody [5G11]

anti-Desmoglein 3 antibody [5G11] for IHC-Formalin-fixed paraffin-embedded sections,SDS-PAGE,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [5G11] recognizes Desmoglein 3
Tested Reactivity Hu
Tested Application IHC-P, SDS-PAGE, WB
Host Mouse
Clonality Monoclonal
Clone 5G11
Isotype IgG1, kappa
Target Name Desmoglein 3
Antigen Species Human
Immunogen Recombinant protein corresponding to the extracellular portion of Human Desmoglein 3.
Conjugation Un-conjugated
Full Name desmoglein 3
Alternate Names PVA; 130 kDa pemphigus vulgaris antigen; CDHF6; Cadherin family member 6; Desmoglein-3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
SDS-PAGEAssay-dependent
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1830 Human DSG3

Swiss-port # P32926 Human Desmoglein-3

Gene Symbol DSG3
Gene Full Name desmoglein 3
Background Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq, Jul 2008]
Function Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. [UniProt]
Cellular Localization Cell surface. [UniProt]
Calculated MW 108 kDa

Images (2) Click the Picture to Zoom In

  • ARG58558 anti-Desmoglein 3 antibody [5G11] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin stained with ARG58558 anti-Desmoglein 3 antibody [5G11]. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min.

  • ARG58558 anti-Desmoglein 3 antibody [5G11] SDS-PAGE image

    SDS-PAGE: Analysis of ARG58558 anti-Desmoglein 3 antibody [5G11]. Confirmation of integrity and purity of the antibody.

    2 µg loading.
    NR: Non-reduced
    R: Reduced.